ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (1): 138-143.doi: 10.11843/j.issn.0366-6964.2015.01.018

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The Influence of Lamotrigine and L-NNA on the Content of Nitric Oxide and the Activity Nitric Oxide Synthase and nNOS Positive Neurons Morphology in the Temporal Lobe Epilepsy Rabbit Induced by Pilocarpine

LI Meng-meng,YIN Xun-he*，QIU Jian-hua，WANG Xian-long，LI Nan   

  1. (College of Animal Science and Technology，Shandong Agricultural University，Tai’an 271018，China)
  • Received:2014-05-23 Online:2015-01-23 Published:2015-01-23

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Abstract:

 By measuring the content of Nitric Oxide and the Activity of Nitric Oxide synthase， this experiment was conducted to study the influence of lamotrigine and L-NNA on the change and investigate the mechanism of the action of NO on the cerebral hemorrhageand the protective effect of those two pharmaceuticals in the temporal lobe epilepsy rabbit induced by pilocarpine.Sixty healthy Haerbin rabbits，2.0-2.5 kg，were divided into the group of physiological saline，the epilepsy model group，and the treatment group of lamotrigine and L-NNA.Nitric Oxide （NO） content and the activity of NOS in the hippocampus and temporal lobe after epilepsy were determined by using nitrate enzyme reduction method.We found that the activity of NOS increased at 6 hours after epilpsy， and increased to the highest at 1 day， then decreased gradually and basically returned to normal level after 7 days，but it was still higher significantly than that of other groups （P<0.05）.The level of NOS in two therapy groups between L-NNA and lamotrigine were lower than that in model group at every time extremely notable difference or notable difference （P<0.01 or P<0.05），and the decreasing level of NOS in L-NNA group was lower than that of group of lamotrigine between 6 h to 1 day （P<0.05）.The change of the NO content and the activity of NOS were accordant primitively and directly correlation.SABC immunohistochemical method was used to detect the neuronal NOS positive neurons in hippocampus，the density of neuronal NOS positive neurons in hippocampus’ CA3 region increased，and the cytoplasm stained darker， soma-sectional area and the length of tuber became smaller; After the treatment of lamotrigine and L-NNA，the density of neuron decreased，and the soma-sectional area and the length of tuber became significant longer （P<0.05）.The results indicate that NO is involved in the initiating process of the temporal lobe seizures，and the high concentration of NO can damage the neurons；L-NNA and the novel therapeutic drug lamotrigine can inhibit the concentration of NO after epilepsy seizures，which have a better therapeutic effect on the epilepsy seizures.

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